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M9490499.TXT
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1994-09-24
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Document 0499
DOCN M9490499
TI Virucidal effect of myeloperoxidase on human immunodeficiency virus type
1-infected T cells.
DT 9411
AU Chochola J; Yamaguchi Y; Moguilevsky N; Bollen A; Strosberg AD;
Stanislawski M; Laboratoire d'Immunologie, Institut de Recherches
Scientifiques; sur le Cancer, Villejuif, France.
SO Antimicrob Agents Chemother. 1994 May;38(5):969-72. Unique Identifier :
AIDSLINE MED/94346849
AB Myeloperoxidase is virucidal to human immunodeficiency virus type 1
(HIV-1) in the persistently infected CEM human T-cell line or in acutely
infected human peripheral blood mononuclear cells, as judged by viral
infectivity and P24 radioimmunoassay. HIV-1 was specifically inactivated
by low doses of the human myeloperoxidase (1.4 to 14.3 mU/ml) and the
cells were spared. A higher enzyme concentration (143 mU/m) was
cytotoxic, but uninfected CEM cells and normal lymphocytes were
resistant to > or = 143 mU of myeloperoxidase per ml. The enzyme was
virucidal with the Cl- present in medium and did not require exogenous
H2O2. Catalase, an antioxidant enzyme, partially inhibited the virucidal
effect of myeloperoxidase. Hence, the H2O2 probably came from the
HIV-infected cells themselves. These in vitro findings indicate that the
myeloperoxidase system is capable of inactivating HIV-1 of infected
cells.
DE Antiviral Agents/*PHARMACOLOGY Catalase/PHARMACOLOGY Cell Line
Glucose Oxidase/PHARMACOLOGY Human HIV Core Protein
p24/IMMUNOLOGY/METABOLISM HIV-1/*DRUG EFFECTS/METABOLISM
Lactoperoxidase/PHARMACOLOGY Peroxidase/*PHARMACOLOGY
Phytohemagglutinins/PHARMACOLOGY Radioimmunoassay Support, Non-U.S.
Gov't T-Lymphocytes/DRUG EFFECTS/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).